cbb3 oxidase has 4 subunits NOPQ, total residues 1129, total
protein mass 124 kDa
P is related to (or equal to?) N-fixing gene Fix-P
Subunit N is homologous to cyt aa3 oxidase subunit 1 (22% I)
(Cytochrome aa3 cytochrome oxidase has 12 transmembrane
helices)
13 predicted here, one may be tmh
N (532 res, 58,956 Da, PI 9.43):
10
20 30
40 50
60
MWDYVKLVALGVVAAIAAYAASQARDLPYMVNMVEVALAAVIALIWVLRTMGDPKPSKDE
YFDGVIRAGVIATTFWGIVGFLVAVVIAFQLAFPALNLEFGNGMLNFGRLRPLHTSAVIF
AFGGNALIASAFYVVQRTSAARLFGGTALGWFVFWGWQLIIVTAATSYLLGGSQGKEYAE
LNWHLDILVAVVWVAYLIAFLGTIFKRKEPHIYVANWFYLSFIVTIAMLHIVNNLAVPVS
IFGTKSVQLMAGVQDAMTQWWYGHNAVGFFLTAGFLGMMYYFVPKQAERPVYSYKLSIVH
FWALIFLYIWAGPHHLHYTALPDWASTLGMVMSVILWMPSWGGMINGLMTLSGAWDKLRT
DPVIRMMVVSIGFYGMSTFEGPMMSIKAVNSLSHYTDWTIGHVHSGALGWNGMITFGMLY
FLTPRLWGRSGLYSLKLVSWHFWLATIGIVLYASAMWVTGIMEGLMWREVDAQGFLVNAF
ADTVAAKFPMYVVRGVGGVLYLLGGLIMAYNLWATVAKQPKTANLAVAVPAE
O - no homology outside cbb3 oxidases.
(242 res, 27,068 Da, PI 4.73)
10
20 30
40 50
60
MSIMDKHHVLEKNATLLLIFAFLVVTIGGIVEIAPLFYLENTIEKVEGMRPYTPLELTGR -60
DIYIREGCYVCHSQMIRPMRDEVERYGHYSLAAESMYDRPFQWGSKRTGPDLARVGGRYS
-120
DAWHVEHLINPQSVVPESVMPSYGYLAKVPLDSTWIEDRVSTDAFVGVPYTSEMIASAKA -180
DFVAQADPNADSTTLLAGYGEKVNIRNFDGQPGLTEMDALVAYLQVLGTMVDFSTFQPVA -240
SR
P - (297 res 31,899 kDa, PI 4.63)
has homology to solved structures 1AOF (Nitrite reductase)
and 1CYJ (cyt c6)
10
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40 50
60
MSKKPTTKKEVQTTGHQWDGIEELNTPLPRWWLWTFYATIIWGVAYSIAMPAWPIFSDKA
TPGLLGSSTRADVEKDIAKFAEMNKAVEEKLVATDLTAIAADPELVTYTRNAGAAVFRTW
CAQCHGAGAGGNTGFPSLLDGDWLHGGAIETIYTNVKHGIRDPLDPDTLLVANMPAHLTD
ELLEPAQIDEVVQYVLQISGQPADEVKATAGQQIFAENCASCHGEDAKGLVEMGAPNLTD
GIWLYGGDVATLTSTIQYGRGGVMPSWSWAADGAKPRLSEAQIRAVASYVHSLGGGQ
Q (58 residues 6546 Da PI 6.0)
10
20 30
40 50
60
MDYHILREFADSWAALALLLTFIGAVIWAFRPGSSKVHDDIANIPFRHEDKPADHGRG