MLSVAARSGPFAPYLSAAAHAVPGPLKALA 30
PAALRAEKVVLDLKRPLLCRESMSGRSARRDLVAGISLNA 70
PASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKG 34
FSYLVTATACVATAYAAKNVVTQFISSLSASADVLALSKI 74
EIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEINQEAEVDV 114
SKLRDPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDFG 154
LVGVCTHLGCVPIANSGDFGGYYCPCHGSHYDASGRIRKG 174
PAPYNLEVPTYQFVGDDLVVVG0
Nucleic acid sequence:
ATGTTGTCCGTGGCCGCCCGCTCCGGGCCCT
TCGCGCCCTACCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGC
CGGCGGCGCTGCGGCCCGAGAAGGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGTCGGG
AGTCGATGAGCGGCCGCTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCAC
CTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTC
GTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGT
TTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTG
TCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAA-AGATTG
AGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAAC
CCCTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGT
CTAAGCTGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATAT
TAGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTG
GTTATTACTGCCCGTGCCATGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTC
CTGCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTTGTTGGTGATGATTTAGTTGTT
GTTGGCTGA
Consensus sequence of the gene with AA seq aligned:
10 20 30 40 50 60
| | | | | |
Cons. NAseq: CGGCGGTGACCGGCGCACCCGCACCCGCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCT
Protein seq: M L S V A A R S G P10
70 80 90 100 110 120
| | | | | |
Cons. NAseq: TCGCGCCCTACCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGC
Protein seq: F A P Y L S A A A H A V P G P L K A L A30
130 140 150 160 170 180
| | | | | |
Cons. NAseq: CGGCGGCGCTGCGGCCCGAGAAGGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGTCGGG
Protein seq: P A A L R A E K V V L D L K R P L L C R50
| | | | | |
Cons. NAseq: AGTCGATGAGCGGCCGCTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCAC
Protein seq: E S M S G R S A R R D L V A G I S L N A70
250 260 270 280 290 300
| | | | | |
Cons. NAseq: CTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTC
Protein seq: P A S V R Y V H N D V T V P D F S A Y R14
310 320 330 340 350 360
| | | | | |
Cons. NAseq: GTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGT
Protein seq: R E D V M D A T T S S Q T S S E D R K G34
370 380 390 400 410 420
| | | | | |
Cons. NAseq: TTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTG
Protein seq: F S Y L V T A T A C V A T A Y A A K N V54
430 440 450 460 470 480
| | | | | |
Cons. NAseq: TCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTG
Protein seq: V T Q F I S S L S A S A D V L A L S K I74
490 500 510 520 530 540
| | | | | |
Cons. NAseq: AGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAAC
Protein seq: E I K L S D I P E G K N V A F K W R G K94
550 560 570 580 590 600
| | | | | |
Cons. NAseq: CCCTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGT
Protein seq: P L F V R H R T Q A E I N Q E A E V D V114
610 620 630 640 650 660
| | | | | |
Cons. NAseq: CTAAGCTGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATAT
Protein seq: S K L R D P Q H D L D R V K K P E W V I134
670 680 690 700 710 720
| | | | | |
Cons. NAseq: TAGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTG
Protein seq: L V G V C T H L G C V P I A N S G D F G154
730 740 750 760 770 780
| | | | | |
Cons. NAseq: GTTATTACTGCCCGTGCCATGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTC
Protein seq: G Y Y C P C H G S H Y D A S G R I R K G174
790 800 810 820 830 840
| | | | | |
Cons. NAseq: CTGCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTTGTTGGTGATGATTTAGTTGTT
Protein seq: P A P Y N L E V P T Y Q F V G D D L V V194
850 860 870 880 890 900
| | | | | |
Cons. NAseq: GTTGGCTGAATAACAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTAA
Protein seq: V G 0
---
Individual nucleic acid sequences used in the alignment:
Chicken ISP sequence determined in Berkeley-
ATGTTGTCCGTGGCCGCCCGCTCCGGGCCCTTC
GCGCCCTACCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGCCGGCGGCGCTGCGGGCCGAGAA
GGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGCCGGGAGTCGATGAGCGGCCGCTCGGCGCGGCGGGATCTCGTCGCCG
GCATCAGCCTCAACGCACCTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGT
GAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCTGGTGACTGCAAC
AGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGC
TAGCATTGTCAAAGATTGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCC
CTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGCTGAGAGATCCACAGCA
CGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTA
ACTCTGGAGATTTTGGTGGTTATTACTGCCCGTGCCATGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCT
GCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTTGTTGGTGATGATTTAGTTGTTGTTGGCTGAATAACAAAGTGCTT
GCTCACTTTCATGTTCTGGTGAATGTATACTTAAAAGGGTTAACTGATATTCTGAAATACTGTCAAACCAGATGATCCTA
AAAATACATCAGCTTCATTCTCTACCGGAAGTATGTTTTGAATACTT
Translated in Frame+1
MLSVAARSGPFAPYLSAAAHAVPGPLKALAPAALRAEKVVLDLKRPLLCRESMSGRSARR
DLVAGISLNAPASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATAC
VATAYAAKNVVTQFISSLSASADVLALSKIEIKLSDIPEGKNVAFKWRGKPLFVRHRTQA
EINQEAEVDVSKLRDPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDFGGYYCPCHGSH
YDASGRIRKGPAPYNLEVPTYQFVGDDLVVVG0ITKCLLTFMFW0MYT0KG0LIF0NTVK
PDDPKNTSASFSTGSMF0IL
http://www.chick.umist.ac.uk/cgi-bin/chickEST.cgi?show_seq=603133842F1
>603133842F1 cloneID='ChEST114d11' 16 days embryo brain
CTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTC
GTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGAAAAGGGT
TTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTG
TCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTG
AGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAAC
CCCTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGT
CTAAGCTGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATAT
TAGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTG
GTTATTACTGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTC
CTGCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTTGTTGGTGATGATTTAGTTGTTG
TTGGCTGAATAACAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTAAAAGG
GTTAACTGATATTCTGAAATACTGTCAAACCAGATGATCCTAAACATACATCAGCTTCAT
TCTCTACCGGAAGTATGTNTGAATACTTCCCTATGTTCAGTTTTAATAAAAACTTGAAGT
GAAAAA
Translated in Frame +3
ASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATACVATAYAAKNVV
TQFISSLSASADVLALSKIEIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEINQEAEVDVS
KLRDPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDFGGYYCPCHGSHYDASGRIRKGP
APYNLEVPTYQFVGDDLVVVG0ITKCLLTFMFW0MYT0KG0LIF0NTVKPDDPKHTSASF
STGSMXEYFPMFSFNKNLK0K
http://www.chick.umist.ac.uk/cgi-bin/chickEST.cgi?show_seq=603112225F1
>603112225F1 cloneID='ChEST60b7' stage 36 hearts
TTGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGT
GATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCT
GGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTT
TATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTGAGATCAAGCT
ATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTTTGT
GCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGCTGAG
AGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAGGAGT
CTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTGGTTATTACTG
CCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCTGCCCCCTA
TAACCTGGAGGTTCCAACCTACCAGTNTGTTGGTGATGATTTAGTTGTTGTTGGCTGAAT
AACAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTAAAAGGGTTAACTGAT
ATTCTGAAATACTGTCAAACCAGATGATCCTAAAAATACATCAGCTTCATTCTCTACCGG
AAGTATGTTGAATACTTCCCTATGTTCAGTTTTAATAAAAACTTGAGGTGGGACAAA
Translated in Frame +2
CYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATACVATAYAAKNVVTQF
ISSLSASADVLALSKIEIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEINQEAEVDVSKLR
DPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDFGGYYCPCHGSHYDASGRIRKGPAPY
NLEVPTYQXVGDDLVVVG0ITKCLLTFMFW0MYT0KG0LIF0NTVKPDDPKNTSASFSTG
SMLNTSLCSVLIKT0GGT
http://www.chick.umist.ac.uk/cgi-bin/chickEST.cgi?show_seq=603540546F1
>603540546F1 cloneID='ChEST509k11' adult heart
GTGCGGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGA
TGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGAAAAGGGTTTTCCTA
CCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCA
GTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTGAGATCAA
GCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTT
AGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGCT
GAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAGG
AGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTGGTGGTTATTAC
TGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCTGCCCCC
TATAACCTGGAGGTTCCAAACCTACCAGTTTGTTGGTGATGATTTAGTTGTGTTGGCTGA
ATAACAAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTAAAAGGGTTAACT
GATATTCTGAAATACTGTCAAAACCAAGATGATCCTAAAAATACCATCAGCTTCATTCTC
TTACTGGAAGTATGTTTGATATACTTCCCCTTATGTTCAGTTTTAAATTAAAAACTTGGA
AGTAAAAA
Translated in Frame +2
CGYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATACVATAYAAKNVVTQ
FISSLSASADVLALSKIEIKLSDIPEGKNVAFKWRGKPLLVRHRTQAEINQEAEVDVSKL
RDPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDLVVITARATGPIMMPLAESGKVLPP
ITWRFQTYQFVGDDLVVLAE0QKCLLTFMFW0MYT0KG0LIF0NTVKTKMILKIPSASFS
YWKYV0YTSPYVQF0IKNLEVK
http://www.chick.umist.ac.uk/cgi-bin/chickEST.cgi?show_seq=603126639F1
>603126639F1 cloneID='ChEST96n9' stage 22 limbs
TGACGGCGCACCCGCACCCGCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCTTCGCGCC
CTACCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGCCGGCGGC
GCTGCGGGCCGAGAAGGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGCCGGGAGTCGAT
GAGCGGCCGCTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCACCTGCCAG
TGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGA
TGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGAAAAGGGTTTTCCTA
CCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCA
GTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAACAGATTGAGATCA
AGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCT
TTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGC
TGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAG
GGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTGGTGGTTATT
ACTGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCTGCCC
CCTATAACCTGGAGGTTCCAAGCCTACCAGCTTTGTGGGTGATGAATTAGGTGGTGGTGG
GCTGAATTACAAAAGTGCTTGCTCACTTTCATGTTCCGGTGAATGTTTTACTTAAAGGGT
TACCTGATATCTGAAATCTGTCAACCCAGATGATCTAAAATTCTTCAGTTTCATTCTCTC
CGAAGTTGTTGAATATCC
Translated in Frame +2
DGAPAPAMLSVAARSGPFAPYLSAAAHAVPGPLKALAPAALRAEKVVLDLKRPLLCRESM
SGRSARRDLVAGISLNAPASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSY
LVTATACVATAYAAKNVVTQFISSLSASADVLALSTD0DQAI0HS0RQKRGFQVERETPL
CASQNPSRD0PGS0S0CV0AERSTARFRQSKETGMGHISRESALILVVYQLLTLEIWWLL
LPVPRVPL0CLWQNQERSCPL0PGGSKPTSFVGDELGGGGLNYKSACSLSCSGECFT0RV
T0YLKSVNPDDLKFFSFILSEVVEY
http://www.chick.umist.ac.uk/cgi-bin/chickEST.cgi?show_seq=603775723F1
>603775723F1 cloneID='ChEST716m6' chicken ovary
CACCGCACNGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCC
TACCGTCGTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGA
AAAGGGTTTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAG
AATGTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAA
AGATNGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAG
GGAAACCCCTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTG
ATGTGTCTAAGCTGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGG
TCATATTAGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATT
TGGTGGTTATTACTGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAA
AGGTCCTGCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTGTTGGTGATGATTTAGTT
GTTGTTGGCTGAATAACAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTAA
AAGGGTTAACTGATATTCTGAAA
Translated in Frame +3
PHXQCALRPQ0CHGP0LLCLPS0RCDGCHNVFSNQQ0RQKRVFLPGDCNSMRSYCICC0E
CVTQFISSLSASADVLALSKXEIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEINQEAEVD
VSKLRDPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDLVVITARATGPIMMPLAESGK
VLPPITWRFQPTSCW00FSCCWLNNKVLAHFHVLVNVYLKGLTDILK
http://www.chick.umist.ac.uk/cgi-bin/chickEST.cgi?show_seq=603117438F1
>603117438F1 cloneID='ChEST73l9' stage 36 trunks
CGGCGGTGACCGGCGCACCCGCACCCGCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCT
TCGCGCCCTACCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGC
CGGCGGCGCTGCGGCCCGAGAAGGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGTCGGG
AGTCGATGAGCGGCCGCTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCAC
CTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTC
GTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGT
TTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTG
TCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCA
Translated in Frame +3
PHXQCALRPQ0CHGP0LLCLPS0RCDGCHNVFSNQQ0RQKRVFLPGDCNSMRSYCICC0E
CVTQFISSLSASADVLALSKXEIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEINQEAEVD
VSKLRDPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDLVVITARATGPIMMPLAESGK
VLPPITWRFQPTSCW00FSCCWLNNKVLAHFHVLVNVYLKGLTDILK
http://www.chick.umist.ac.uk/cgi-bin/chickEST.cgi?show_seq=603112661F1
>603112661F1 cloneID='ChEST61j15' stage 36 hearts
GTTCGCACCCGCACCCGCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCTTCGCGCCCTA
CCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGCCGGCGGCGCT
GCGGCCCGAGAAGGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGTCGGGAGTCGATGAG
CGGCCGCTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCACCTGCCAGTGT
GCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGT
GATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCT
GGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTT
TATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCA
Translated in Frame +2
FAPAPAMLSVAARSGPFAPYLSAAAHAVPGPLKALAPAALRPEKVVLDLKRPLLCRESMS
GRSARRDLVAGISLNAPASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYL
VTATACVATAYAAKNVVTQFISSLSASADVLALS
http://www.chick.umist.ac.uk/cgi-bin/chickEST.cgi?show_seq=603112578F1
>603112578F1 cloneID='ChEST61l20' stage 36 hearts
TTCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCTTCGCGCCCTACCTGTCGGCCGCGGC
GCACGCCGGGCCCGGCCCGCTCAAGGCGCTGGCGCCGGCGGCGCTGCGGCCCGAGAAGGT
GGTGCTGGACCTGAAGCGACCGCTGCTGTGTCGGGAGTCGATGAGCGGCCGCTCGGCGCG
GCGGGATCTCGTCGCCGGCATCAGCCTCAACGCACCTGCCAGTGTGCGTTACGTCCACAA
TGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCCACAAC
GTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCTGGTGACTGCAACAGC
ATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAG
TGCTTCTGCTGATGTGCTAGCATTGTCA
Translated in Frame +2
SMLSVAARSGPFAPYLSAAAHAGPGPLKALAPAALRPEKVVLDLKRPLLCRESMSGRSAR
RDLVAGISLNAPASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATA
CVATAYAAKNVVTQFISSLSASADVLALS
http://www.chick.umist.ac.uk/cgi-bin/chickEST.cgi?show_seq=603109719F1
>603109719F1 cloneID='ChEST54f6' stage 36 hearts
GCGGCTCGAGGCGGCTCGAGGCGGCTCGAGCACCTGCCAGTGTGCGTTACGTCCACAATG
ATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCCACAACGT
CTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCTGGTGACTGCAACAGCAT
GCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAGTG
CTTCTGCTGATGTGCTAGCATTGTCA
Translated in Frame +3
GSRRLEAARAPASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATAC
VATAYAAKNVVTQFISSLSASADVLALS
http://www.chick.umist.ac.uk/cgi-bin/chickEST.cgi?show_seq=603152431F1
>603152431F1 cloneID='ChEST161m8' chicken muscle
CTTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCACCTGCCAGTGTGCGTT
ACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGG
ATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCTGGTGA
CTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTT
CCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCA
Translated in Frame +3
SARRDLVAGISLNAPASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVT
ATACVATAYAAKNVVTQFISSLSASADVLALS
http://www.chickest.udel.edu/public_php/getSequence.php?seqId=77857&type=standard
>pgl1n.pk012.j20
CGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGAAAAGGGTTTTCCTACCT
GGTGACTGTAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCA
TTGTCAAAGATTGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTTTGTGCGTCACAGAACCC
AAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGCTGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATT
AGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTGGTTATTACTGCCCGTGCCACGGGTCCCATTATGATGCC
TCTGGCAGAATCAGGAAAGGTCCTGCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTTGTTGGTGATGATTTAGT
Translated in Frame +3
VPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTVTACVATAYAAKNVVTQFISSLSASA
DVLALSKIEIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEINQEAEVDVSKLRDPQHDLDR
VKKPEWVILVGVCTHLGCVPIANSGDFGGYYCPCHGSHYDASGRIRKGPAPYNLEVPTYQ
FVGDDL
http://www.chickest.udel.edu/public_php/getSequence.php?seqId=60358&type=standard
>pat.pk0053.g8.f Frame +2
CATCAGCCTCAACGCACCTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCC
ACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATG
TTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAA
CGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGCTGAGA
GATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAGGAGTCTGCACTCATCTTGGTGTGTACAATTGCTAACTCTGGA
GATTTGGTGGTATTACTGCCGTGCATGGGTCCATTATGATCCTCTGGCAGAATCAGGAAAGTCTGCCCCTATAACTGGNGGTCAACTACANTTTGTG
GGGATGATTAGTTGTTGTGGNGAAAAA
Translated in Frame +2
ISLNAPASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATACVATAY
AAKNVVTQFISSLSASADVLALSKIEIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEINQE
AEVDVSKLRDPQHDLDRVKKPEWVILVGVCTHLGVYNC0LWRFGGITAVHGSIMILWQNQ
ESLPL0LXVNYXLWG0LVVVXK
http://www.chickest.udel.edu/public_php/getSequence.php?seqId=57622&type=standard
>pat.pk0008.e6 Frame -3
TTTTTTTTTTTTTCACTTCAAGTTTTTATTAAAACTGAACATAGGGAAGTATTCAAACATACTTCCGGTAGAGAATGAAGCTGATGTATTTTTAGGA
TCATCTGGTTTGACAGTATTTCAGAATATCAGTTAACCCTTTTAAGTATACATTCACCAGAACATGAAAGTNAGCAAGCACTTTGTTATTCAGCCAA
CAACAACTAAATCATCACCAACAAACTGGTAGGTTGGAACCTCCAGGTTATAGGGGGCAGGACCTTTCCTGATTCTGCCAGAGGCATCATAATGGGA
CCCGTGGCACGGGCAGTAATAACCACCAAAATCTCCAGAGTTAGCAATTGGTACACAACCAAGATGAGTGCAGACTCCTACTAATATGACCCATTCC
GGTTTCTTTACTCTGTCTAAATCGTGCTGTGGATCTCTCAGCTTAGACACATCAACTTCANTTCCTGGTAACTCTGCTTGGGTCTGTGACNACAAAG
AGGGTTCCT
Translated in Frame -3
EPSLXSQTQAELPGXEVDVSKLRDPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDFGG
YYCPCHGSHYDASGRIRKGPAPYNLEVPTYQFVGDDLVVVG0ITKCLXTFMFW0MYT0KG
0LIF0NTVKPDDPKNTSASFSTGSMFEYFPMFSFNKNLK0KKKK
http://www.chickest.udel.edu/public_php/getSequence.php?seqId=89373&type=standard
>pgm2n.pk008.o8 Frame -1
AAGTCAGGAACAAGTGCTCACTTCAAGTTTTTATTAAAACTGAACATAGGGAAGTATTCAAACATACTTCCGGTAGAGAATGAAGCTGATGTATTTT
TAAGATCATCTGGTTTGACAGTATTTCAGAATATCAGTTAACCCTTTTAAGTATACATTCACCAGAACATGAAAGTGAGCAAGCACTTTGTTATTCA
GCCAACAACAACTAAATCATCACCAACAAACTGGTAGGTTGGAACCTCCAGGTTATAGGGGGCAGGACCTTTCCTGATTCTGCCAGAGGCATCATAA
TGGGACCCGTGGCACGGGCAGTAATAACCACCAAAATCTCCAGAGTTAGCAATTGGTACACAACCAAGATGAGTGCAGACTCCTACTAATATGACCC
ATTCCGGTTTCTTTACTCTGTCTAAATCGTGCTGTGGATCTCTCAGCTTNNNCACATCAACTTCAGCTTCCTGGTTAATCTCTGCTTGGGTTCTGTG
ACGCACAAAGAGGGGTTTCCCTCTCCACTTGAAAGCCACGTTTTTGCCTTCAGGAATGTCAGATAGCTTGATCTCAATCTTTGACAATGCTAGCACA
Translated in Frame -1:
TCAGCAGAAGCACTGAGGCTGGAAATAAACTGGGTGACAACATTCTT
KNVVTQFISSLSASADVLALSKIEIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEINQEAE
VDVXKLRDPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDFGGYYCPCHGSHYDASGRI
RKGPAPYNLEVPTYQFVGDDLVVVG0ITKCLLTFMFW0MYT0KG0LIF0NTVKPDDLKNT
SASFSTGSMFEYFPMFSFNKNLK0ALVPD
http://www.chickest.udel.edu/public_php/getSequence.php?seqId=60393&type=standard
>pat.pk0054.c10.f
CTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGAAAAGGGTTTTCCTACCTGGTGAC
TGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCA
AAGATTGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTTTGTGCGTCACAGAACCCAAGCAG
AGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAAGCTGAGAGATCACAGCACGATTAGACAGATTAAGAAACGGAATGGTCATATANTAGGATCNC
ACCACCTTGGTGTGTCAATTGCAACCCGGAATTTTGGGGTATNCNCCNTGCACGGTCCATANATCCCNGCAAACAGAAAGGCTGCCCCANACCTGAG
GTCAACNCATTGTGGGANATTATGTGTGGTGA
Translated in Frame +3
DFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATACVATAYAAKNVVTQFISSLSASADV
LALSKIEIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEINQEAEVDVSKAERSQHD0TD0E
TEWSYXRIXPPWCVNCNPEFWGXXXARSIXPXKQKGCPXPEVNXLWXIMCG
One more?
pat.pk0064.d2.f length=636 84 1e-15
-------------------------------------------------------------
three sequences good at C-term: pgm2npk008o8 patpk0008e6 603133842F1 603112225F1
pat.pk0054.c10.f only good for ~150 bases in middle, tends to misalign
but aligns correctly with berkeley+ pgm2npk008o8 603133842F1 603112225F1?
five sequences good around N-term of mature:
603112661F1 603117438F1 603112578F1 603152431F1 603109719F1
603112661F1 603117438F1 603112578F1 extend almost to N-term of precursor
603126639F1 covers entire range but poor sequence everywhere.
FASTA format for alignment (reverse compliment for negative-frame sequences):
>berkeleyISP
ATGTTGTCCGTGGCCGCCCGCTCCGGGCCCTTC
GCGCCCTACCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGCCGGCGGCGCTGCGGGCCGAGAA
GGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGCCGGGAGTCGATGAGCGGCCGCTCGGCGCGGCGGGATCTCGTCGCCG
GCATCAGCCTCAACGCACCTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGT
GAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCTGGTGACTGCAAC
AGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGC
TAGCATTGTCAAAGATTGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCC
CTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGCTGAGAGATCCACAGCA
CGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTA
ACTCTGGAGATTTTGGTGGTTATTACTGCCCGTGCCATGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCT
GCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTTGTTGGTGATGATTTAGTTGTTGTTGGCTGAATAACAAAGTGCTT
GCTCACTTTCATGTTCTGGTGAATGTATACTTAAAAGGGTTAACTGATATTCTGAAATACTGTCAAACCAGATGATCCTA
AAAATACATCAGCTTCATTCTCTACCGGAAGTATGTTTTGAATACTT
>603133842F1 cloneID='ChEST114d11' 16 days embryo brain Frame 3
CTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTC
GTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGAAAAGGGT
TTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTG
TCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTG
AGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAAC
CCCTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGT
CTAAGCTGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATAT
TAGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTG
GTTATTACTGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTC
CTGCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTTGTTGGTGATGATTTAGTTGTTG
TTGGCTGAATAACAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTAAAAGG
GTTAACTGATATTCTGAAATACTGTCAAACCAGATGATCCTAAACATACATCAGCTTCAT
TCTCTACCGGAAGTATGTNTGAATACTTCCCTATGTTCAGTTTTAATAAAAACTTGAAGT
GAAAAA
>603540546F1 cloneID='ChEST509k11' adult heart
GTGCGGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGA
TGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGAAAAGGGTTTTCCTA
CCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCA
GTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTGAGATCAA
GCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTT
AGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGCT
GAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAGG
AGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTGGTGGTTATTAC
TGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCTGCCCCC
TATAACCTGGAGGTTCCAAACCTACCAGTTTGTTGGTGATGATTTAGTTGTGTTGGCTGA
ATAACAAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTAAAAGGGTTAACT
GATATTCTGAAATACTGTCAAAACCAAGATGATCCTAAAAATACCATCAGCTTCATTCTC
TTACTGGAAGTATGTTTGATATACTTCCCCTTATGTTCAGTTTTAAATTAAAAACTTGGA
AGTAAAAA
>pgl1n.pk012.j20 Frame +3
CGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGAAAAGGGTTTTCCTACCT
GGTGACTGTAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCA
TTGTCAAAGATTGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTTTGTGCGTCACAGAACCC
AAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGCTGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATT
AGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTGGTTATTACTGCCCGTGCCACGGGTCCCATTATGATGCC
TCTGGCAGAATCAGGAAAGGTCCTGCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTTGTTGGTGATGATTTAGT
>pat.pk0054.c10.f Length: 517 Standard View
CTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCA
GCAGTGAAGACAGAAAAGGGTTTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTG
CATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATG
TGCTAGCATTGTCAAAGATTGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGG
CTTTCAAGTGGAGAGGGAAACCCCTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACC
AGGAAGCTGAAGTTGATGTGTCTAAAGCTGAGAGATCACAGCACGATTAGACAGATTAAG
AAACGGAATGGTCATATANTAGGATCNCACCACCTTGGTGTGTCAATTGCAACCCGGAAT
TTTGGGGTATNCNCCNTGCACGGTCCATANATCCCNGCAAACAGAAAGGCTGCCCCANAC
CTGAGGTCAACNCATTGTGGGANATTATGTGTGGTGA
>pat.pk0053.g8.f Frame +2
CATCAGCCTCAACGCACCTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCC
ACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATG
TTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAA
CGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGCTGAGA
GATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAGGAGTCTGCACTCATCTTGGTGTGTACAATTGCTAACTCTGGA
GATTTGGTGGTATTACTGCCGTGCATGGGTCCATTATGATCCTCTGGCAGAATCAGGAAAGTCTGCCCCTATAACTGGNGGTCAACTACANTTTGTG
GGGATGATTAGTTGTTGTGGNGAAAAA
>603775723F1 cloneID='ChEST716m6' chicken ovary
CACCGCACNGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCC
TACCGTCGTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGA
AAAGGGTTTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAG
AATGTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAA
AGATNGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAG
GGAAACCCCTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTG
ATGTGTCTAAGCTGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGG
TCATATTAGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATT
TGGTGGTTATTACTGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAA
AGGTCCTGCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTGTTGGTGATGATTTAGTT
GTTGTTGGCTGAATAACAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTAA
AAGGGTTAACTGATATTCTGAAA
>603126639F1 cloneID='ChEST96n9' stage 22 limbs
TGACGGCGCACCCGCACCCGCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCTTCGCGCC
CTACCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGCCGGCGGC
GCTGCGGGCCGAGAAGGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGCCGGGAGTCGAT
GAGCGGCCGCTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCACCTGCCAG
TGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGA
TGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGAAAAGGGTTTTCCTA
CCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCA
GTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAACAGATTGAGATCA
AGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCT
TTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGC
TGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAG
GGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTGGTGGTTATT
ACTGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCTGCCC
CCTATAACCTGGAGGTTCCAAGCCTACCAGCTTTGTGGGTGATGAATTAGGTGGTGGTGG
GCTGAATTACAAAAGTGCTTGCTCACTTTCATGTTCCGGTGAATGTTTTACTTAAAGGGT
TACCTGATATCTGAAATCTGTCAACCCAGATGATCTAAAATTCTTCAGTTTCATTCTCTC
CGAAGTTGTTGAATATCC
>pgm2n.pk008.o8
AAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTGAGATCAAGCTATCTGACATTCCTGAAG
GCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTTTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGNNNAA
GCTGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCT
AACTCTGGAGATTTTGGTGGTTATTACTGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCTGCCCCCTATAACCTGG
AGGTTCCAACCTACCAGTTTGTTGGTGATGATTTAGTTGTTGTTGGCTGAATAACAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTA
AAAGGGTTAACTGATATTCTGAAATACTGTCAAACCAGATGATCTTAAAAATACATCAGCTTCATTCTCTACCGGAAGTATGTTTGAATACTTCCCT
ATGTTCAGTTTTAATAAAAACTTGAAGTGAGCACTTGTTCCTGACTT
>pat.pk0008.e6 Frame -3
AGGAACCCTCTTTGTNGTCACAGACCCAAGCAGAGTTACCAGGAANTGAAGTTGATGTGTCTAAGCTGAGAGATCCACAGCACGATTTAGACAGAGT
AAAGAAACCGGAATGGGTCATATTAGTAGGAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTGGTTATTACTGCCCG
TGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCTGCCCCCTATAACCTGGAGGTTCCAACCTACCAGTTTGTTGGTGATGATT
TAGTTGTTGTTGGCTGAATAACAAAGTGCTTGCTNACTTTCATGTTCTGGTGAATGTATACTTAAAAGGGTTAACTGATATTCTGAAATACTGTCAA
ACCAGATGATCCTAAAAATACATCAGCTTCATTCTCTACCGGAAGTATGTTTGAATACTTCCCTATGTTCAGTTTTAATAAAAACTTGAAGTGAAAA
AAAAAAAAA
>603112225F1 cloneID='ChEST60b7' stage 36 hearts frame 2
TTGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGT
GATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCT
GGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTT
TATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTGAGATCAAGCT
ATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTTTGT
GCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGCTGAG
AGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAGGAGT
CTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTGGTTATTACTG
CCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCTGCCCCCTA
TAACCTGGAGGTTCCAACCTACCAGTNTGTTGGTGATGATTTAGTTGTTGTTGGCTGAAT
AACAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTAAAAGGGTTAACTGAT
ATTCTGAAATACTGTCAAACCAGATGATCCTAAAAATACATCAGCTTCATTCTCTACCGG
AAGTATGTTGAATACTTCCCTATGTTCAGTTTTAATAAAAACTTGAGGTGGGACAAA
>603112578F1 cloneID='ChEST61l20' stage 36 hearts
TTCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCTTCGCGCCCTACCTGTCGGCCGCGGC
GCACGCCGGGCCCGGCCCGCTCAAGGCGCTGGCGCCGGCGGCGCTGCGGCCCGAGAAGGT
GGTGCTGGACCTGAAGCGACCGCTGCTGTGTCGGGAGTCGATGAGCGGCCGCTCGGCGCG
GCGGGATCTCGTCGCCGGCATCAGCCTCAACGCACCTGCCAGTGTGCGTTACGTCCACAA
TGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCCACAAC
GTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCTGGTGACTGCAACAGC
ATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAG
TGCTTCTGCTGATGTGCTAGCATTGTCA
>603109719F1 cloneID='ChEST54f6' stage 36 hearts
GCGGCTCGAGGCGGCTCGAGGCGGCTCGAGCACCTGCCAGTGTGCGTTACGTCCACAATG
ATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCCACAACGT
CTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCTGGTGACTGCAACAGCAT
GCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAGTG
CTTCTGCTGATGTGCTAGCATTGTCA
>603152431F1 cloneID='ChEST161m8' chicken muscle
CTTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCACCTGCCAGTGTGCGTT
ACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGG
ATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCTGGTGA
CTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTT
CCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCA
>603112661F1 cloneID='ChEST61j15' stage 36 hearts
GTTCGCACCCGCACCCGCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCTTCGCGCCCTA
CCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGCCGGCGGCGCT
GCGGCCCGAGAAGGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGTCGGGAGTCGATGAG
CGGCCGCTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCACCTGCCAGTGT
GCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTCGTGAAGATGT
GATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGTTTTCCTACCT
GGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTT
TATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCA
>603117438F1 cloneID='ChEST73l9' stage 36 trunks
CGGCGGTGACCGGCGCACCCGCACCCGCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCT
TCGCGCCCTACCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGC
CGGCGGCGCTGCGGCCCGAGAAGGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGTCGGG
AGTCGATGAGCGGCCGCTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCAC
CTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTC
GTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGT
TTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTG
TCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCA
>pat.pk0054.c10.f Frame +3
CTGACTTCTCTGCCTACCGTCGTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGACAGAAAAGGGTTTTCCTACCTGGTGAC
TGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTGTCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCA
AAGATTGAGATCAAGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCTTTGTGCGTCACAGAACCCAAGCAG
AGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAAGCTGAGAGATCACAGCACGATTAGACAGATTAAGAAACGGAATGGTCATATANTAGGATCNC
ACCACCTTGGTGTGTCAATTGCAACCCGGAATTTTGGGGTATNCNCCNTGCACGGTCCATANATCCCNGCAAACAGAAAGGCTGCCCCANACCTGAG
GTCAACNCATTGTGGGANATTATGTGTGGTGA
Prim.cons.
CGGCGGTGACCGGCGCACCCGCACCCGCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCT
TCGCGCCCTACCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGC
CGGCGGCGCTGCGGCCCGAGAAGGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGTCGGG
AGTCGATGAGCGGCCGCTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCAC
CTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTC
GTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGT
TTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTG
TCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAA-AGATTGAGATCA
AGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCT
TTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGC
TGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAG
G-AGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTGGTTAT
TACTGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCTGCC
CCCTATAACCTGGAGGTTCCAA-CCTACCAG-TTTGTTGGTGATGATTTAGTTGTTGTTG
G-CTGAATAACAAA-GTGCTTGCTCACTTTCATGTTCTGGTGAATGTAT-ACTTAAAAGG
GTTAACTGATATTCTGAAATACTGTCAAACCAGATGATCCTAAAAATACATCAGCTTCAT
TCTCTACCGGAAGTATGT-TTGAATACTTCCCTATGTTCAGTTTTAATAAAAACTTGAA
>Prim.cons. with discarded inserts removed
CGGCGGTGACCGGCGCACCCGCACCCGCCATGTTGTCCGTGGCCGCCCGCTCCGGGCCCT
TCGCGCCCTACCTGTCGGCCGCGGCGCACGCCGTGCCCGGCCCGCTCAAGGCGCTGGCGC
CGGCGGCGCTGCGGCCCGAGAAGGTGGTGCTGGACCTGAAGCGACCGCTGCTGTGTCGGG
AGTCGATGAGCGGCCGCTCGGCGCGGCGGGATCTCGTCGCCGGCATCAGCCTCAACGCAC
CTGCCAGTGTGCGTTACGTCCACAATGATGTCACGGTCCCTGACTTCTCTGCCTACCGTC
GTGAAGATGTGATGGATGCCACAACGTCTTCTCAAACCAGCAGTGAAGATAGAAAAGGGT
TTTCCTACCTGGTGACTGCAACAGCATGCGTAGCTACTGCATATGCTGCTAAGAATGTTG
TCACCCAGTTTATTTCCAGCCTCAGTGCTTCTGCTGATGTGCTAGCATTGTCAAAGATTGAGATCA
AGCTATCTGACATTCCTGAAGGCAAAAACGTGGCTTTCAAGTGGAGAGGGAAACCCCTCT
TTGTGCGTCACAGAACCCAAGCAGAGATTAACCAGGAAGCTGAAGTTGATGTGTCTAAGC
TGAGAGATCCACAGCACGATTTAGACAGAGTAAAGAAACCGGAATGGGTCATATTAGTAG
GAGTCTGCACTCATCTTGGTTGTGTACCAATTGCTAACTCTGGAGATTTTGGTGGTTAT
TACTGCCCGTGCCACGGGTCCCATTATGATGCCTCTGGCAGAATCAGGAAAGGTCCTGCC
CCCTATAACCTGGAGGTTCCAACCTACCAGTTTGTTGGTGATGATTTAGTTGTTGTTG
GCTGAATAACAAAGTGCTTGCTCACTTTCATGTTCTGGTGAATGTATACTTAAAAGG
GTTAACTGATATTCTGAAATACTGTCAAACCAGATGATCCTAAAAATACATCAGCTTCAT
TCTCTACCGGAAGTATGTTTGAATACTTCCCTATGTTCAGTTTTAATAAAAACTTGAA
>Prim.cons. translated in Frame 3:
AVTGAPAPAMLSVAARSGPFAPYLSAAAHAVPGPLKALAPAALRPEKVVLDLKRPLLCRE
SMSGRSARRDLVAGISLNAPASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGF
SYLVTATACVATAYAAKNVVTQFISSLSASADVLALSKIEIKLSDIPEGKNVAFKWRGKP
LFVRHRTQAEINQEAEVDVSKLRDPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDFGG
YYCPCHGSHYDASGRIRKGPAPYNLEVPTYQFVGDDLVVVG0ITKCLLTFMFW0MYT0KG
0LIF0NTVKPDDPKNTSASFSTGSMFEYFPMFSFNKNL
>Berkeley Frame 0
MLSVAARSGPFAPYLSAAAHAVPGPLKALAPAALRAEKVVLDLKRPLLCRESMSGRSARR
DLVAGISLNAPASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATAC
VATAYAAKNVVTQFISSLSASADVLALSKIEIKLSDIPEGKNVAFKWRGKPLFVRHRTQA
EINQEAEVDVSKLRDPQHDLDRVKKPEWVILVGVCTHLGCVPIANSGDFGGYYCPCHGSH
YDASGRIRKGPAPYNLEVPTYQFVGDDLVVVG0ITKCLLTFMFW0MYT0KG0LIF0NTVK
PDDPKNTSASFSTGSMF0IL
39F sequence:
>39F Frame+1
0RRTRTRHVVRGRPLRALRALPVGRGARRARPAQGAGAGGAAGREGGAGPEATAAVPGVD
ERPLGAAGSRRRHQPQRTCQCALRPQ0CHGPxLLCLPSxRCDGCHNVFSNQQ0RQKRVFL
PGDCNSMRSYCICCxECCHPVYFQPQCFC0CASIVNRLRSSYLTFLKAKTWLSSGEGNPS
LCVTEPKQRLTRKLKLMCLS0EIHSTI0TE0RNRNGSYxxGVCTHLGCVPIANSGDLVVI
TARATGPIMMPLAESGKVLPPITWRFQAYQLCGxxIRWWWAELQKCLLTFMFRxMFYLKG
YLISEICQPR0SKILQFHSLRSC0IS
>39F Frame+2 (8-156, A36->P)
DGAPAPAMLSVAARSGPFAPYLSAAAHAVPGPLKALAPAALRAEKVVLDLKRPLLCRESM
SGRSARRDLVAGISLNAPASVRYVHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSY
LVTATACVATAYAAKNVVTQFISSLSASADVLALSTDxDQAIxHSxRQKRGFQVERETPL
CASQNPSRDxPGSxSxCVxAERSTARFRQSKETGMGHISRESALILVVYQLLTLEIWWLL
LPVPRVPLxCLWQNQERSCPLxPGGSKPTSFVGDELGGGGLNYKSACSLSCSGECFTxRV
TxYLKSVNPDDLKFFSFILSEVVEY
>Frame+3
TAHPHPPCCPWPPAPGPSRPTCRPRRTPCPARSRRWRRRRCGPRRWCWTxSDRCCAGSRx
AAARRGGISSPASASTHLPVCVTSTMMSRSLTSLPTVVKMxWMPQRLLKPAVKTEKGFPT
WxLQQHAxLLHMLLRMLSPSLFPASVLLLMCxHCQQIEIKLSDIPEGKNVAFKWRGKPLF
VRHRTQAEINQEAEVDVSKLRDPQHDLDRVKKPEWVILVGSLHSSWLCTNCxLWRFGGYY
CPCHGSHYDASGRIRKGPAPYNLEVPSLPALWVMNxVVVGxITKVLAHFHVPVNVLLKGL
PDIxNLSTQMIxNSSVSFSPKLLNI