20                                                        30
                          |         |         |         |         |
              I      R      K    W    Y     Y     N     A     A     G     F      N     K    Y      G     L      M    R     D     D
603590745F1   TTCGAAAGTGGTACTATAATGCGGCTGGATTCAACAAATACGGATTAATGCGAGATGATA

                                                    60                                                         70
                          |         |         |         |         |
                                      N     E      R     M    F      R     I       K    R      A     L     D     L      S     L      K     H     R      I      L
603590745F1   ATGAAAGAATGTTTCGCATAAAGAGAGCACTTGATTTAAGCTTGAAACATCGGATCCTTC
 

                                                    80                                                         90
                          |         |         |         |         |
                                      P      K     E     Q     W    V      K     Y    E    E    D       K       P     Y      L     E      P     Y      L      K
603590745F1   CTAAAGAACAGTGGGTAAAATATGAAGAGGATAAGCCTTATCTTGAACCTTACCTAAAAG
 

                          100                           110
                          |         |         |         |         |
                                      E     V      I      R      E      R     L      E     R      E     A     W   N      K     K  X
603590745F1   AAGTAATCCGTGAACGACTTGAAAGAGAAGCATGGAACAAGAAATAACTATTGAACTATT
 

                                                    120                                                       130
                          |         |         |         |         |
603590745F1   TTGTGCAAATAGCCACATCTTTCTGATATTTCTAAAGTTCGGTTATTCAAGTCTTCAGGG
 

                                                    140                                                       150
                          |         |         |         |         |
603590745F1   AAGCACATGTAAGGCTGAAGGAACACTCGTTTCAGCTTGTTCATTGAGTCTGTTCGTCCA
 

                                                     160                                                      170
                          |         |         |         |         |
603590745F1   ACTAAAATAAATATGTCAGAGTATAAAATCACTTGGGAAGGCTTCTTTATAAATGTTAGC
 

                                                    180                                                       190
                          |         |         |         |         |
603590745F1   AAGCTAATGTTCAGATCTTTCCTATGAGCATATACATTACACATTAGCTTAATGGTATGT
 

                                                    200                                                       210
                          |         |         |         |         |
603590745F1   AGTCTTCATTCCAGCTTCTTGATTCTTACTTCTTTCATAGTACAGCTGTTTCCTGTAGTT
 

                                                    220
                          |         |         |         |         |
603590745F1   AGAGGGTTAGGTAGGAACACACCCAGAATTGCTGGTTAAGTTGTTAACTTAAACAAGGGA
 
 

2.Sub6 Protein Alignment
X = termination codon
red color = high homology
blue = homology
green = homology w/ mutation
- = Alignment not there. Sequence out of Frame. Maybe insert.
 

  10        20        30        40        50        60
                       |         |         |         |         |         |
603851052F1   ----AARATVAGGGRLMDRIRKWYYNAAGFNKYGLMRDDTLYEDDDVKEALKRLPEDLYN
603593392F1   ----AARATVAGGGRLMDRIRKWYYNAAGFNKYGLMRDDTLYEDDDVKEALKRLPEDLYN
603590745F1   EVKMAARATVAGGGRLMDRIRKWYYNAAGFNKYGLMRDDTLYEDDDVKEALKRLPEDLYN
                  ********************************************************
Prim.cons.    EVKMAARATVAGGGRLMDRIRKWYYNAAGFNKYGLMRDDTLYEDDDVKEALKRLPEDLYN

                      70        80        90       100       110       120
                       |         |         |         |         |         |
6X3851X52F1   ERMFRIKRALDLSLKHRILPKEQWVKYEEDKPYLEPYLKEVIRERLEREAWNKKXLLNYF
6X3593392F1   ERMFRIKRALDLSLKHRILPKEQWVKYEEDKPYLEPYLKEVIRERLEREAWNKKXLLNYF
6X359X745F1   ERMFRIKRALDLSLKHRILPKEQWVKYEEDKPYLEPYLKEVIRERLEREAWNKKXLLNYF
            ************************************************************
Prim.cons.    ERMFRIKRALDLSLKHRILPKEQWVKYEEDKPYLEPYLKEVIRERLEREAWNKKXLLNYF

                     130       140       150       160       170       180
                       |         |         |         |         |         |
6X3851X52F1   VHIATSFXYFXSSVIQVFREAHVRLKEYSFQLVHXVCSSNXNKYVRVXNHLGRLLYKCXQ
6X3593392F1   VQIATSFXYFXSSVIQVFREAHVRLKEHSFQLXHXVCSSNXNKYVRVXNHLGRLLYKCXQ
6X359X745F1   VQIATSFXYFXSSVIQVFREAHVRLKEHSFQLVHXVCSSNXNKYVRVXNHLGRLLYKCXQ
            *:*************************:**** ***************************
Prim.cons.    VQIATSFXYFXSSVIQVFREAHVRLKEHSFQLVHXVCSSNXNKYVRVXNHLGRLLYKCXQ

                     190       200       210       220       230       240
                       |         |         |         |         |         |
6X3851X52F1   ANVQIFPMSIYITHXLNGVXSSFQLLDSYFFHSTAVSCSXRVRXETP-------------
6X3593392F1   ANVQIFPMSIYITHXLNGMXSSFQLLDSYFFHSTAVSCSXRVRXETPRIAGXVVNFNKGG
6X359X745F1   ANVQIFPMSIYITHXLNGMXSSFQLLDSYFFHSTAVSCSXRVRXEHTQNCWLSCXLKQGT
            ******************:************************** .
Prim.cons.    ANVQIFPMSIYITHXLNGMXSSFQLLDSYFFHSTAVSCSXRVRXETP22222222222G2
 
 
 
 

3.Subunit 6 alignment DNA with Amino Acid Sequence
Red: Complete Homology
- : Alignment not there. Sequence out of Frame. Maybe insert.
X: stop

10        20        30        40        50        60
                       |         |         |         |         |         |
603590745F1   CCGAGGTCAAGATGGCGGCGAGGGCAACGGTTGCAGGAGGTGGTCGCCTGATGGACAGAA
603851052F1   --------------GCGGCGAGGGCAACGGTTGCAGGAGGTGGTCGCCTGATGGACAGAA
603593392F1   ------------TGGCGGCGAGGGCAACGGTTGCAGGAGGTGGTCGCCTGATGGACAGAA
                            **********************************************
Prim.cons.    CCGAGGTCAAGATGGCGGCGAGGGCAACGGTTGCAGGAGGTGGTCGCCTGATGGACAGAA

                      70        80        90       100       110       120
                       |         |         |         |         |         |
603590745F1   TTCGAAAGTGGTACTATAATGCGGCTGGATTCAACAAATACGGATTAATGCGAGATGATA
603851052F1   TTCGAAAGTGGTACTATAATGCGGCTGGATTCAACAAATATGGATTAATGCGAGATGATA
603593392F1   TTCGAAAGTGGTACTATAATGCGGCTGGATTCAACAAATACGGATTAATGCGAGATGATA
            **************************************** *******************
Prim.cons.    TTCGAAAGTGGTACTATAATGCGGCTGGATTCAACAAATACGGATTAATGCGAGATGATA

                     130       140       150       160       170       180
                       |         |         |         |         |         |
603590745F1   CATTGTATGAAGATGATGATGTCAAAGAAGCACTGAAGAGACTTCCAGAGGATCTTTACA
603851052F1   CATTGTATGAAGATGATGATGTCAAAGAAGCACTGAAGAGACTTCCAGAGGATCTTTACA
603593392F1   CATTGTATGAAGATGATGATGTCAAAGAAGCACTGAAGAGACTTCCAGAGGATCTTTACA
            ************************************************************
Prim.cons.    CATTGTATGAAGATGATGATGTCAAAGAAGCACTGAAGAGACTTCCAGAGGATCTTTACA
              T  L  Y  E  D  D  D  V  K  E  A  L  K  R  L  P  E  D  L  Y

                     190       200       210       220       230       240
                       |         |         |         |         |         |
603590745F1   ATGAAAGAATGTTTCGCATAAAGAGAGCACTTGATTTAAGCTTGAAACATCGGATCCTTC
603851052F1   ATGAAAGAATGTTTCGCATAAAGAGAGCACTTGATTTAAGCTTGAAACATCGGATCCTTC
603593392F1   ATGAAAGAATGTTTCGCATAAAGAGAGCACTTGATTTAAGCTTGAAACATCGGATCCTTC
            ************************************************************
Prim.cons.    ATGAAAGAATGTTTCGCATAAAGAGAGCACTTGATTTAAGCTTGAAACATCGGATCCTTC

                     250       260       270       280       290       300
                       |         |         |         |         |         |
603590745F1   CTAAAGAACAGTGGGTAAAATATGAAGAGGATAAGCCTTATCTTGAACCTTACCTAAAAG
603851052F1   CTAAAGAACAGTGGGTAAAATATGAAGAGGATAAGCCTTATCTTGAACCTTACCTAAAAG
603593392F1   CTAAAGAACAGTGGGTAAAATATGAAGAGGATAAGCCTTATCTTGAACCTTACCTAAAAG
            ************************************************************
Prim.cons.    CTAAAGAACAGTGGGTAAAATATGAAGAGGATAAGCCTTATCTTGAACCTTACCTAAAAG

                     310       320       330       340       350       360
                       |         |         |         |         |         |
603590745F1   AAGTAATCCGTGAACGACTTGAAAGAGAAGCATGGAACAAGAAATAACTATTGAACTATT
603851052F1   AAGTAATCCGTGAACGACTTGAAAGAGAAGCATGGAACAAGAAATAACTATTGAACTATT
603593392F1   AAGTAATCCGTGAACGACTTGAAAGAGAAGCATGGAACAAGAAATAACTATTGAACTATT
            ************************************************************
Prim.cons.    AAGTAATCCGTGAACGACTTGAAAGAGAAGCATGGAACAAGAAATAACTATTGAACTATT

                     370       380       390       400       410       420
                       |         |         |         |         |         |
603590745F1   TTGTGCAAATAGCCACATCTTTCTGATATTTCTAAAGTTCGGTTATTCAAGTCTTCAGGG
603851052F1   TTGTGCATATAGCCACATCTTTCTGATATTTCTAAAGTTCGGTTATTCAAGTCTTCAGGG
603593392F1   TTGTGCAAATAGCCACATCTTTCTGATATTTCTAAAGTTCGGTTATTCAAGTCTTCAGGG
            ******* ****************************************************
Prim.cons.    TTGTGCAAATAGCCACATCTTTCTGATATTTCTAAAGTTCGGTTATTCAAGTCTTCAGGG

                     430       440       450       460       470       480
                       |         |         |         |         |         |
603590745F1   AAGCACATGTAAGGCTGAAGGAACACTCGTTTCAGCTTGTTCATTGAGTCTGTTCGTCCA
603851052F1   AAGCACATGTAAGGCTGAAGGAATACTCGTTTCAGCTTGTTCATTGAGTCTGTTCGTCCA
603593392F1   AAGCACATGTAAGGCTGAAGGAACACTCGTTTCAGCTTGTNCATTGAGTCTGTTCGTCCA
            *********************** **************** *******************
Prim.cons.    AAGCACATGTAAGGCTGAAGGAACACTCGTTTCAGCTTGTTCATTGAGTCTGTTCGTCCA

                     490       500       510       520       530       540
                       |         |         |         |         |         |
603590745F1   ACTAAAATAAATATGTCAGAGTATAAAATCACTTGGGAAGGCTTCTTTATAAATGTTAGC
603851052F1   ACTAAAATAAATATGTCAGAGTATAAAATCACTTGGGAAGGCTTCTTTATAAATGTTAGC
603593392F1   ACTAAAATAAATATGTCAGAGTATAAAATCACTTGGGAAGGCTTCTTTATAAATGTTAGC
            ************************************************************
Prim.cons.    ACTAAAATAAATATGTCAGAGTATAAAATCACTTGGGAAGGCTTCTTTATAAATGTTAGC

                     550       560       570       580       590       600
                       |         |         |         |         |         |
603590745F1   AAGCTAATGTTCAGATCTTTCCTATGAGCATATACATTACACATTAGCTTAATGGTATGT
603851052F1   AAGCTAATGTTCAGATCTTTCCTATGAGCATATACATTACACATTAGCTTAATGGTGTGT
603593392F1   AAGCTAATGTTCAGATCTTTCCTATGAGCATATACATTACACATTAGCTTAATGGTATGT
            ******************************************************** ***
Prim.cons.    AAGCTAATGTTCAGATCTTTCCTATGAGCATATACATTACACATTAGCTTAATGGTATGT

                     610       620       630       640       650       660
                       |         |         |         |         |         |
603590745F1   AGTCTTCATTCCAGCTTCTTGATTCTTACTTCTTTCATAGTACAGCTGTTTCCTGTAGTT
603851052F1   AGTCTTCATTCCAGCTTCTTGATTCTTACTTCTTTCATAGTACAGCTGTTTCCTGTAGTT
603593392F1   AGTCTTCATTCCAGCTTCTTGATTCTTACTTCTTTCATAGTACAGCTGTTTCCTGTAGTT
            ************************************************************
Prim.cons.    AGTCTTCATTCCAGCTTCTTGATTCTTACTTCTTTCATAGTACAGCTGTTTCCTGTAGTT

                     670       680       690       700       710       720
                       |         |         |         |         |         |
603590745F1   AGAGGGTTAGGTAGGAACACACCCAGAATTGCTGGTTAAGTTGTTAACTTAAACAAGGGA
603851052F1   AGAGGGTTAGGTAGGAA-ACACCCA-----------------------------------
603593392F1   AGAGGGTTAGGTAGGAA-ACACCCAGAATTGCTGGTTAAGTCGTTAACTTTAACAAAGGA
            ***************** *******
Prim.cons.    AGAGGGTTAGGTAGGAACACACCCAGAATTGCTGGTTAAGT2GTTAACTT2AACAA2GGA
 
 

4.Information
 

http://www.chick.umist.ac.uk/cgi-bin/chickEST2.cgi?retrieveESTinfo.x=1
>603590745F1 Frame = +3 st: 1-110 CCGAGGTCAAGATGGCGGCGAGGGCAACGGTTGCAGGAGGTGGTCGCCTGATGGACAGAA
TTCGAAAGTGGTACTATAATGCGGCTGGATTCAACAAATACGGATTAATGCGAGATGATA
CATTGTATGAAGATGATGATGTCAAAGAAGCACTGAAGAGACTTCCAGAGGATCTTTACA
ATGAAAGAATGTTTCGCATAAAGAGAGCACTTGATTTAAGCTTGAAACATCGGATCCTTC
CTAAAGAACAGTGGGTAAAATATGAAGAGGATAAGCCTTATCTTGAACCTTACCTAAAAG
AAGTAATCCGTGAACGACTTGAAAGAGAAGCATGGAACAAGAAATAACTATTGAACTATT
TTGTGCAAATAGCCACATCTTTCTGATATTTCTAAAGTTCGGTTATTCAAGTCTTCAGGG
AAGCACATGTAAGGCTGAAGGAACACTCGTTTCAGCTTGTTCATTGAGTCTGTTCGTCCA
ACTAAAATAAATATGTCAGAGTATAAAATCACTTGGGAAGGCTTCTTTATAAATGTTAGC
AAGCTAATGTTCAGATCTTTCCTATGAGCATATACATTACACATTAGCTTAATGGTATGT
AGTCTTCATTCCAGCTTCTTGATTCTTACTTCTTTCATAGTACAGCTGTTTCCTGTAGTT
AGAGGGTTAGGTAGGAACACACCCAGAATTGCTGGTTAAGTTGTTAACTTAAACAAGGGA
CGTGCTATTTCCTTCATCTCTTCTCCCTGGAGATGAGAATACACGGCCAGTGCTTCATGT GGGTAG

Frame 2 EVKMAARATVAGGGRLMDRIRKWYYNAAGFNKYGLMRDDTLYEDDDVKEALKRLPEDLYN
ERMFRIKRALDLSLKHRILPKEQWVKYEEDKPYLEPYLKEVIRERLEREAWNKK0LLNYF
VQIATSF0YF0SSVIQVFREAHVRLKEHSFQLVH0VCSSN0NKYVRV0NHLGRLLYKC0Q
ANVQIFPMSIYITH0LNGM0SSFQLLDSYFFHSTAVSCS0RVR0EHTQNCWLSC0LKQGT CYFLHLFSLEMRIHGQCFMWV

 http://www.chick.umist.ac.uk/cgi-bin/chickEST2.cgi?retrieveESTinfo.x=1
>603851052F1 Frame = +1 St: 1-110 GCGGCGAGGGCAACGGTTGCAGGAGGTGGTCGCCTGATGGACAGAATTCGAAAGTGGTAC
TATAATGCGGCTGGATTCAACAAATATGGATTAATGCGAGATGATACATTGTATGAAGAT
GATGATGTCAAAGAAGCACTGAAGAGACTTCCAGAGGATCTTTACAATGAAAGAATGTTT
CGCATAAAGAGAGCACTTGATTTAAGCTTGAAACATCGGATCCTTCCTAAAGAACAGTGG
GTAAAATATGAAGAGGATAAGCCTTATCTTGAACCTTACCTAAAAGAAGTAATCCGTGAA
CGACTTGAAAGAGAAGCATGGAACAAGAAATAACTATTGAACTATTTTGTGCATATAGCC
ACATCTTTCTGATATTTCTAAAGTTCGGTTATTCAAGTCTTCAGGGAAGCACATGTAAGG
CTGAAGGAATACTCGTTTCAGCTTGTTCATTGAGTCTGTTCGTCCAACTAAAATAAATAT
GTCAGAGTATAAAATCACTTGGGAAGGCTTCTTTATAAATGTTAGCAAGCTAATGTTCAG
ATCTTTCCTATGAGCATATACATTACACATTAGCTTAATGGTGTGTAGTCTTCATTCCAG
CTTCTTGATTCTTACTTCTTTCATAGTACAGCTGTTTCCTGTAGTTAGAGGGTTAGGTAG GAAACACCCA

Frame 0 AARATVAGGGRLMDRIRKWYYNAAGFNKYGLMRDDTLYEDDDVKEALKRLPEDLYNERMF
RIKRALDLSLKHRILPKEQWVKYEEDKPYLEPYLKEVIRERLEREAWNKK0LLNYFVHIA
TSF0YF0SSVIQVFREAHVRLKEYSFQLVH0VCSSN0NKYVRV0NHLGRLLYKC0QANVQ IFPMSIYITH0LNGV0SSFQLLDSYFFHSTAVSCS0RVR0ETP

http://www.chick.umist.ac.uk/cgi-bin/chickEST2.cgi?retrieveESTinfo.x=11
>603593392F1 Frame = +3 st: 1-110 TGGCGGCGAGGGCAACGGTTGCAGGAGGTGGTCGCCTGATGGACAGAATTCGAAAGTGGT
ACTATAATGCGGCTGGATTCAACAAATACGGATTAATGCGAGATGATACATTGTATGAAG
ATGATGATGTCAAAGAAGCACTGAAGAGACTTCCAGAGGATCTTTACAATGAAAGAATGT
TTCGCATAAAGAGAGCACTTGATTTAAGCTTGAAACATCGGATCCTTCCTAAAGAACAGT
GGGTAAAATATGAAGAGGATAAGCCTTATCTTGAACCTTACCTAAAAGAAGTAATCCGTG
AACGACTTGAAAGAGAAGCATGGAACAAGAAATAACTATTGAACTATTTTGTGCAAATAG
CCACATCTTTCTGATATTTCTAAAGTTCGGTTATTCAAGTCTTCAGGGAAGCACATGTAA
GGCTGAAGGAACACTCGTTTCAGCTTGTNCATTGAGTCTGTTCGTCCAACTAAAATAAAT
ATGTCAGAGTATAAAATCACTTGGGAAGGCTTCTTTATAAATGTTAGCAAGCTAATGTTC
AGATCTTTCCTATGAGCATATACATTACACATTAGCTTAATGGTATGTAGTCTTCATTCC
AGCTTCTTGATTCTTACTTCTTTCATAGTACAGCTGTTTCCTGTAGTTAGAGGGTTAGGT
AGGAAACACCCAGAATTGCTGGTTAAGTCGTTAACTTTAACAAAGGAGGTGCTATTTCCT TCATCTCTTCTCCCTGGAGATGAGAATACGGGGCCAG
Frame 2
AARATVAGGGRLMDRIRKWYYNAAGFNKYGLMRDDTLYEDDDVKEALKRLPEDLYNERMF
RIKRALDLSLKHRILPKEQWVKYEEDKPYLEPYLKEVIRERLEREAWNKK0LLNYFVQIA
TSF0YF0SSVIQVFREAHVRLKEHSFQLXH0VCSSN0NKYVRV0NHLGRLLYKC0QANVQ
IFPMSIYITH0LNGM0SSFQLLDSYFFHSTAVSCS0RVR0ETPRIAG0VVNFNKGGAISF ISSPWR0EYGA